Crop Breeding and Applied Biotechnology (Jan 2009)

Development and validation of SSR markers for Coffea arabica L.

  • Robson Fernando Missio,
  • Eveline Teixeira Caixeta,
  • Eunize Maciel Zambolim,
  • Laércio Zambolim,
  • Ney Sussumu Sakiyama

Journal volume & issue
Vol. 9, no. 4
pp. 361 – 371

Abstract

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With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries withprobes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences.Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were morefrequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1%of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSRmarkers were validated for further genetic studies of C. arabica.

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