Frontiers in Immunology (May 2021)
Analysis of Porcine RIG-I Like Receptors Revealed the Positive Regulation of RIG-I and MDA5 by LGP2
- Shuangjie Li,
- Shuangjie Li,
- Shuangjie Li,
- Shuangjie Li,
- Jie Yang,
- Jie Yang,
- Jie Yang,
- Jie Yang,
- Yuanyuan Zhu,
- Yuanyuan Zhu,
- Yuanyuan Zhu,
- Yuanyuan Zhu,
- Hui Wang,
- Hui Wang,
- Hui Wang,
- Hui Wang,
- Xingyu Ji,
- Xingyu Ji,
- Xingyu Ji,
- Xingyu Ji,
- Jia Luo,
- Jia Luo,
- Jia Luo,
- Jia Luo,
- Qi Shao,
- Qi Shao,
- Qi Shao,
- Qi Shao,
- Yulin Xu,
- Yulin Xu,
- Yulin Xu,
- Yulin Xu,
- Xueliang Liu,
- Xueliang Liu,
- Xueliang Liu,
- Xueliang Liu,
- Wanglong Zheng,
- Wanglong Zheng,
- Wanglong Zheng,
- Wanglong Zheng,
- François Meurens,
- François Meurens,
- Nanhua Chen,
- Nanhua Chen,
- Nanhua Chen,
- Nanhua Chen,
- Jianzhong Zhu,
- Jianzhong Zhu,
- Jianzhong Zhu,
- Jianzhong Zhu
Affiliations
- Shuangjie Li
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Shuangjie Li
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Shuangjie Li
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Shuangjie Li
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jie Yang
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Jie Yang
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jie Yang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Jie Yang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Yuanyuan Zhu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Yuanyuan Zhu
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Yuanyuan Zhu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Yuanyuan Zhu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hui Wang
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Hui Wang
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Hui Wang
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Hui Wang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Xingyu Ji
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Xingyu Ji
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Xingyu Ji
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Xingyu Ji
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jia Luo
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Jia Luo
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jia Luo
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Jia Luo
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Qi Shao
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Qi Shao
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Qi Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Qi Shao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Yulin Xu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Yulin Xu
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Yulin Xu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Yulin Xu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Xueliang Liu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Xueliang Liu
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Xueliang Liu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Xueliang Liu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Wanglong Zheng
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Wanglong Zheng
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Wanglong Zheng
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Wanglong Zheng
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- François Meurens
- INRAE, Oniris, BIOEPAR, Nantes, France
- François Meurens
- Department of Veterinary Microbiology and Immunology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada
- Nanhua Chen
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Nanhua Chen
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Nanhua Chen
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Nanhua Chen
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jianzhong Zhu
- Comparative Medicine Research Institute, Yangzhou University, Yangzhou, China
- Jianzhong Zhu
- College Veterinary Medicine, Yangzhou University, Yangzhou, China
- Jianzhong Zhu
- Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou, China
- Jianzhong Zhu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- DOI
- https://doi.org/10.3389/fimmu.2021.609543
- Journal volume & issue
-
Vol. 12
Abstract
The RLRs play critical roles in sensing and fighting viral infections especially RNA virus infections. Despite the extensive studies on RLRs in humans and mice, there is a lack of systemic investigation of livestock animal RLRs. In this study, we characterized the porcine RLR members RIG-I, MDA5 and LGP2. Compared with their human counterparts, porcine RIG-I and MDA5 exhibited similar signaling activity to distinct dsRNA and viruses, via similar and cooperative recognitions. Porcine LGP2, without signaling activity, was found to positively regulate porcine RIG-I and MDA5 in transfected porcine alveolar macrophages (PAMs), gene knockout PAMs and PK-15 cells. Mechanistically, LGP2 interacts with RIG-I and MDA5 upon cell activation, and promotes the binding of dsRNA ligand by MDA5 as well as RIG-I. Accordingly, porcine LGP2 exerted broad antiviral functions. Intriguingly, we found that porcine LGP2 mutants with defects in ATPase and/or dsRNA binding present constitutive activity which are likely through RIG-I and MDA5. Our work provided significant insights into porcine innate immunity, species specificity and immune biology.
Keywords
