Remineralization of Artificially Demineralized Human Enamel and Dentin Samples by Zinc-Carbonate Hydroxyapatite Nanocrystals
Stefan Kranz,
Markus Heyder,
Stephan Mueller,
André Guellmar,
Christoph Krafft,
Sandor Nietzsche,
Caroline Tschirpke,
Volker Herold,
Bernd Sigusch,
Markus Reise
Affiliations
Stefan Kranz
Department of Conservative Dentistry and Periodontology, Jena University Hospital, Friedrich-Schiller University, An der alten Post 4, 07743 Jena, Germany
Markus Heyder
Department of Conservative Dentistry and Periodontology, Jena University Hospital, Friedrich-Schiller University, An der alten Post 4, 07743 Jena, Germany
Stephan Mueller
Department of Conservative Dentistry and Periodontology, Jena University Hospital, Friedrich-Schiller University, An der alten Post 4, 07743 Jena, Germany
André Guellmar
Department of Conservative Dentistry and Periodontology, Jena University Hospital, Friedrich-Schiller University, An der alten Post 4, 07743 Jena, Germany
Christoph Krafft
Leibniz Institute of Photonic Technology (IPHT), 07745 Jena, Germany
Sandor Nietzsche
Center of Electron Microscopy, Jena University Hospital, Friedrich-Schiller University, 07743 Jena, Germany
Caroline Tschirpke
Otto Schott Institute of Materials Research, Friedrich-Schiller University, 07743 Jena, Germany
Volker Herold
Otto Schott Institute of Materials Research, Friedrich-Schiller University, 07743 Jena, Germany
Bernd Sigusch
Department of Conservative Dentistry and Periodontology, Jena University Hospital, Friedrich-Schiller University, An der alten Post 4, 07743 Jena, Germany
Markus Reise
Department of Conservative Dentistry and Periodontology, Jena University Hospital, Friedrich-Schiller University, An der alten Post 4, 07743 Jena, Germany
(1) Background: Decalcified enamel and dentin surfaces can be regenerated with non-fluoride-containing biomimetic systems. This study aimed to investigate the effect of a zinc carbonate-hydroxyapatite-containing dentifrice on artificially demineralized enamel and dentin surfaces. (2) Methods: Human enamel and dentin discs were prepared and subjected to surface demineralization with 30% orthophosphoric acid for 60 s. Subsequently, in the test group (n = 20), the discs were treated three times a day for 3 min with a zinc carbonate-hydroxyapatite-containing toothpaste (biorepair®). Afterwards, all samples were gently rinsed with PBS (5 s) and stored in artificial saliva until next use. Samples from the control group (n = 20) received no dentifrice-treatment and were stored in artificial saliva, exclusively. After 15 days of daily treatment, specimens were subjected to Raman spectroscopy, energy-dispersive X-ray micro-analysis (EDX), white-light interferometry, and profilometry. (3) Results: Raman spectroscopy and white-light interferometry revealed no significant differences compared to the untreated controls. EDX analysis showed calcium phosphate and silicon dioxide precipitations on treated dentin samples. In addition, treated dentin surfaces showed significant reduced roughness values. (4) Conclusions: Treatment with biorepair® did not affect enamel surfaces as proposed. Minor mineral precipitation and a reduction in surface roughness were detected among dentin surfaces only.
