Stem Cell Research (May 2017)

Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD)

  • Eszter Varga,
  • Csilla Nemes,
  • István Bock,
  • Zsuzsanna Táncos,
  • Sára Berzsenyi,
  • György Lévay,
  • Viktor Román,
  • Julianna Kobolák,
  • András Dinnyés

DOI
https://doi.org/10.1016/j.scr.2017.03.013
Journal volume & issue
Vol. 21, no. C
pp. 19 – 22

Abstract

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Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies.