Investigating the neuroprotective potential of rAAV2‐PCBP1‐EGFP gene therapy against a 6‐OHDA‐induced model of Parkinson's disease

Abstract

Abstract Objectives Previous studies have suggested a potential link between poly(rC)‐binding protein 1 (PCBP1) and neurodegenerative diseases, including Parkinson's disease (PD). However, the precise role of PCBP1 in the pathogenesis of PD remains unclear. Therefore, the main objective of this study was to investigate the neuroprotective effects of PCBP1 in a PD model. Methods To evaluate the neuroprotective potential of PCBP1, we conducted cell count assays and observed the expression of heat shock protein 70 (HSP70) in SH‐SY5Y cells exposed to 6‐OHDA‐induced neurotoxicity. Additionally, we utilized recombinant adeno‐associated virus (rAAV2) vectors encoding PCBP1 or EGFP, which were injected into the rat striatum. After 2 weeks of vector or saline injection, 6‐OHDA was administered to the rat striatum. Behavioral assessments using the open field test (OFT) were performed weekly for 7 weeks. At the seventh week after 6‐OHDA injection, immunohistochemistry and protein expression analyses were conducted in the three groups. Results The results indicated that PCBP1 treatment significantly reduced the proliferation of 6‐OHDA‐induced SH‐SY5Y cells. Additionally, in surviving cells, overexpression of PCBP1 enhanced the expression of HSP70. Similarly, rAAV2 vectors effectively delivered PCBP1 into the brain, resulting in sustained expression of rAAV2‐PCBP1‐EGFP. In the OFT, PCBP1 exhibited significant improvements in behavioral abnormalities and reduced anxiety in the PD model rats (p < .01). Moreover, PCBP1 effectively prevented the decrease of tyrosine hydroxylase and HSP70 expression in the lesioned side induced by 6‐OHDA (p < .01). Consistent with expectations, PCBP1 efficiently protected against cell death caused by 6‐OHDA (p < .01). Conclusions In conclusion, our findings provide compelling evidence for the beneficial effects of PCBP1 in the PD model, suggesting that PCBP1 could be a potential therapeutic target for PD.

Keywords

• 6‐OHDA
• gene therapy
• Parkinson's disease
• poly(rC)‐binding protein 1
• recombinant adeno‐associated virus