Metabolism of long-chain polyunsaturated alcohols in myelinating brain

Abstract

cis-9-[1-14C]Octadecenol, cis,cis-9,12-[1-14C]octadecadienol, and cis,cis,cis-9,12,15-[1-14C]octadecatrienol were administered intracerebrally to 18-day-old rats. Incorporation of radioactivity into the constituent alkyl, alk-1-enyl, and acyl moieties of the ethanolamine phosphatides of brain was determined after 3, 6, 24, and 48 hr. Incorporation of radioactivity from each precursor proceeded at approximately the same rate leading to mono-, di-, and triunsaturated alkyl and alk-1-enyl glycerols. In addition, the labeled alcohols were found to be oxidized to the corresponding fatty acids which were incorporated into acyl groups; radioactivity derived from di- and triunsaturated alcohols was found mainly in acyl moieties produced through chain elongation and desaturation reactions of di- and triunsaturated fatty acids.

Keywords

• [1-14C]octadecenol
• [1-14C]-octadecadienol
• [1-14C]octadecatrienol
• intracerebral injection
• ethanolamine phosphatides
• alkyl acyl glycerols