Protective action of priming agents on Urochloa brizantha seeds under water restriction and salinity conditions

Abstract

Abstract: Analyses of germination of seeds under artificial stress conditions are tools for a better understanding of the survival and adaptation capacity of species under natural stress conditions. Therefore, the aim of this study was to evaluate the effect of priming on seed germination, on breaking of dormancy, and on the length of Urochloa brizantha seedlings under water restriction and salt stress conditions. A completely randomized experimental design was used with four replications in a 3 × 6 factorial arrangement consisting of 3 germination conditions (water, water restriction with PEG, and salinity with NaCl) and 6 treatments [five priming solutions (distilled water, potassium nitrate, spermidine, gibberellin, and sodium nitroprusside) and a control (unprimed seeds)]. The following determinations were made: seed moisture content after priming (before drying) and after drying the seeds, germination test at seven and twenty-one days after sowing, germination speed index, viability by the tetrazolium test of the seeds remaining from the germination test, and seedling length. Priming is effective in minimizing the negative effects of water restriction and salinity. Use of sodium nitroprusside as a priming solution results in a lower percentage of dormant seeds and greater germination, vigor, and plant development.

Keywords

• brachiaria
• sodium nitroprusside
• spermidine
• water stress
• salt stress