Scientific Reports (Sep 2021)

A gain series method for accurate EMCCD calibration

  • Duncan P. Ryan,
  • Megan K. Dunlap,
  • Martin P. Gelfand,
  • James H. Werner,
  • Alan K. Van Orden,
  • Peter M. Goodwin

DOI
https://doi.org/10.1038/s41598-021-97759-6
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Abstract Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.