Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin

Jing-Ying Chen; Ying-Sui Sun; Diem Thuy Nguyen; Chia-Fei Liu; Her-Hsiung Huang

Materials & Design (Nov 2022)

Surface characterizations and cell growth of rough zirconia surfaces immobilized with different BMP-2 concentrations using natural crosslinker genipin

Jing-Ying Chen,
Ying-Sui Sun,
Diem Thuy Nguyen,
Chia-Fei Liu,
Her-Hsiung Huang

Affiliations

Jing-Ying Chen: Department of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Ying-Sui Sun: Department of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; School of Dental Technology, Taipei Medical University, Taipei 110, Taiwan
Diem Thuy Nguyen: Department of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Chia-Fei Liu: Department of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan
Her-Hsiung Huang: Department of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Institute of Oral Biology, National Yang Ming Chiao Tung University, Taipei 112, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung 413, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei 112, Taiwan; Department of Education and Research, Taipei City Hospital, Taipei 103, Taiwan; Corresponding author at: Department of Dentistry, National Yang Ming Chiao Tung University, No.155, Sec.2, Li-Nong Street, Taipei 112, Taiwan.

Journal volume & issue: Vol. 223
p. 111216

Abstract

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Zirconia (ZrO2) is widely used in the dental implant industry, due to its good mechanical properties and biocompatibility. However, the bioinert nature of ZrO2 may hinder osseointegration. This study investigated the responses of human bone marrow mesenchymal stem cells (hMSCs) to sandblasted ZrO2 surfaces immobilized with bone morphogenetic protein-2 (BMP-2) using the natural crosslinker genipin. The immobilization of BMP on sandblasted ZrO2 surfaces increased the surface roughness but did not alter the wettability. The focal adhesion activation and later-stage mineralization of hMSCs were higher on BMP-2-modified ZrO2 surfaces than on untreated surfaces. The BMP-2-modified ZrO2 surfaces with low (100 ng/mL) and high (500 ng/mL) concentrations showed no significant difference in later-stage osteogenic response. However, ZrO2 surfaces with low BMP-2 concentration earlier stimulated the p38 (mitogen-activated protein kinase pathway) protein expression, osteogenic early marker osteopontin expression, and later-stage mineralization. This simple but effective process to immobilize BMP-2 on rough ZrO2 surfaces can benefit future dental implant applications.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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