Nature Communications (Mar 2020)

The ribosome-associated complex RAC serves in a relay that directs nascent chains to Ssb

  • Ying Zhang,
  • Genís Valentín Gesé,
  • Charlotte Conz,
  • Karine Lapouge,
  • Jürgen Kopp,
  • Tina Wölfle,
  • Sabine Rospert,
  • Irmgard Sinning

DOI
https://doi.org/10.1038/s41467-020-15313-w
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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The ribosome-associated complex (RAC), which contains the Hsp40 protein Zuo1 and the non-canonical Hsp70 protein Ssz1 forms a chaperone triad with the fungal-specific Hsp70 protein Ssb. Here the authors combine X-ray crystallography, crosslinking and biochemical experiments and present the structure of the Zuo1 N-terminus bound to Ssz1 and demonstrate that Ssz1 is an active chaperone for nascent chains.