Frontiers in Marine Science (Dec 2024)
Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation
- Yacheng Hu,
- Yacheng Hu,
- Ruihua Tan,
- Yang Li,
- Yang Li,
- Tingting Shu,
- Tingting Shu,
- Gang Yang,
- Zhong Chu,
- Haoran Wang,
- Xueqing Liu,
- Xueqing Liu,
- Xin Zhu,
- Xin Zhu,
- Binzhong Wang,
- Binzhong Wang,
- Baifu Guo,
- Baifu Guo,
- Runqiu Wang,
- Runqiu Wang,
- Huantao Qu,
- Huantao Qu,
- Haoyong Song,
- Haoyong Song,
- Dezhi Zhang,
- Dezhi Zhang,
- Hejun Du,
- Hejun Du,
- Zhiyuan Li,
- Zhiyuan Li,
- Jianbo Chang,
- Jianbo Chang,
- Dianchang Wang,
- Dan Xu,
- Wei Jiang,
- Wei Jiang,
- Qingfei Li,
- Yuanjin Yang,
- Yuanjin Yang
Affiliations
- Yacheng Hu
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Yacheng Hu
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Ruihua Tan
- National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
- Yang Li
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Yang Li
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Tingting Shu
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Tingting Shu
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Gang Yang
- School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Zhong Chu
- School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Haoran Wang
- School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Xueqing Liu
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Xueqing Liu
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Xin Zhu
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Xin Zhu
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Binzhong Wang
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Binzhong Wang
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Baifu Guo
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Baifu Guo
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Runqiu Wang
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Runqiu Wang
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Huantao Qu
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Huantao Qu
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Haoyong Song
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Haoyong Song
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Dezhi Zhang
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Dezhi Zhang
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Hejun Du
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Hejun Du
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Zhiyuan Li
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Zhiyuan Li
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Jianbo Chang
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Jianbo Chang
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Dianchang Wang
- China Three Gorges Corporation, Wuhan, Hubei, China
- Dan Xu
- National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China
- Wei Jiang
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Wei Jiang
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- Qingfei Li
- School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Yuanjin Yang
- Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, China
- Yuanjin Yang
- Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, China
- DOI
- https://doi.org/10.3389/fmars.2024.1506932
- Journal volume & issue
-
Vol. 11
Abstract
IntroductionOur understanding of the regulatory mechanisms that govern the development of sexual characteristics in sturgeon, particularly the Chinese sturgeon (Acipenser sinensis), is limited. Foxl2, a member of the forkhead/HNF-3-related transcription factor family, is known for its role in precise gene regulation within specific tissues and its control over ovarian aromatase, a key enzyme in ovarian development and maturation. This study aimed to investigate the sexually dimorphic expression of foxl2 during the molecular and morphological sex differentiation stages in Chinese sturgeon.MethodsWe successfully isolated the full-length cDNAs of foxl2 in Chinese sturgeon. We then examined the expression profiles of this gene across various somatic tissues in both female and male specimens.ResultsFoxl2 was predominantly expressed in the ovulatory organs of two-year-old Chinese sturgeon, with significantly lower expression in other somatic tissues. We observed sexual dimorphism in foxl2 expression within gonads that had achieved morphological differentiation.DiscussionThe results suggest that foxl2 expression is intricately linked to the process of ovarian differentiation in Chinese sturgeon. Furthermore, we propose that the mRNA quantities of foxl2 could serve as an accurate biomarker for sex determination in sturgeon during their early growth stages.
Keywords
