口腔疾病防治 (May 2017)

Expression of autophagy⁃related proteins PTEN and MAP1LC3 in tongue squamous cell carcinoma

  • WANG Ya⁃nan,
  • LIU Hao,
  • ZHANG Jun

DOI
https://doi.org/10.12016/j.issn.2096⁃1456.2017.05.004
Journal volume & issue
Vol. 25, no. 5
pp. 294 – 299

Abstract

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Objective This study aims to evaluate the expression of autophagy⁃related protein cancer phosphatase and tensinhomology deleted on chromosome ten (PTEN) and microtubule⁃associated protein 1 light chain 3 (MAP1LC3) and investigate its significance in tongue squamous cell carcinoma (TSCC). Methods About 46 TSCC samples, 13 cas⁃ es of precancerous lesion and 13 cases of adjacent tissues were enrolled. Immunohistochemistry were performed to ex⁃ amine the expression of PTEN, MAP1LC3. Statistical analyses were carried out to assess the associations among clinic pathologic parameters. Results The positive expression rate of PTEN (34.78%) in TSCC was significantly lower than that in adjacent tissues (69.23%, c2 = 4.926, P = 0.026) and precancerous lesions (76.92%, c2 = 7.302, P = 0.007). The positive expression rate of MAP1LC3 (28.26%) in TSCC was significantly lower than that of adjacent tissues (61.53%,c2 = 4.896, P = 0.027) and precancerous lesions (69.23%, c2 = 7.275, P = 0.007), the difference was statistically signifi⁃ cant. The expression of PTEN and MAP1LC3 was not correlated with age, sex, smoking, alcohol consumption and cell differentiation (P > 0.05). There was a significant difference in the expression of PTEN and MAP1LC3 in subgroup with different clinical stage and in subgroup with or without lymph node metastasis (P < 0.05). There is a significant differ⁃ ence in the tumor recurrence between PTEN protein expression positive subgroup and PTEN protein expression nega⁃ tive subgroup (c2 = 4.629，P = 0.039), and there was no significant difference in the tumor recurrence between MAP1LC3 protein expression positive subgroup and MAP1LC3 protein expression negative subgroup (c2 = 0.343，P = 0.453). There was a positive correlation between PTEN and MAP1LC3. Conclusion The expression of PTEN and MAP1LC3 suggested that autophagy associated proteins play a pivotal role in the progression, diagnosis and prognosis of TSCC. Down⁃regulation of PTEN and MAP1LC3 was observed in TSCC.

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