The effect of nettle extract on antioxidant defense system in piglets after weaning

Abstract

Background. The effect of common nettle (Urtica dioica L.) extracts on the free radical processes and antioxidant system in piglets during the critical period of weaning from sows has been studied. Materials and Methods. Large white piglets were divided into 2 groups (control and experimental), 9 animals in each. Piglets of the experimental group from 14 days of age and before weaning received the standard diet and the nettle extract in the dose of 6 mg/kg of body weight for 22 days. The blood, as well as erythrocyte hemolysates and plasma of piglets obtained at 14, 36, and 42 days of age, were studied. Results. Our results have shown that weaning causes an oxidative stress in piglets. This state leads to an increase in the concentration of metabolites of free radical damage to protein molecules – carbonyl groups of proteins on the first day and primary products of lipid peroxidation on the seventh day after weaning. This activation of oxidative damage occurs in piglets against the background of a physiologically immature antioxidant system and is characterized by a decrease in the activity of the enzymatic chain – superoxide dismutase, glutathione peroxidase and catalase, as well as the concentration of its non-enzymatic antioxidant – reduced glutathione. Feeding piglets with nettle extract leads to activation of the antioxidant defense system in erythrocytes (higher activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and an increased reduced glutathione level compared to the control values) and a decrease in the concentration of oxidative damage products in the plasma (the content of lipid hydroperoxides, TBA-active products and carbonyl groups of proteins). Conclusion. The positive effect of nettle extract on the inhibition of free radical processes and activation of antioxidant systems indicates that this extract can be added to the standard diet of young animals to increase stress resistance and adaptability of their organism in critical periods of ontogenesis.

Keywords

• antioxidant system
• oxidative damage products
• nettle extract
• piglets
• weaning stress