The chemokine Cxcl14 regulates interneuron differentiation in layer I of the somatosensory cortex
Andrew F. Iannone,
Gülcan Akgül,
Robin Zhang,
Sam Wacks,
Nisma Hussein,
Carmen Ginelly Macias,
Alexander Donatelle,
Julia M.J. Bauriedel,
Cora Wright,
Debra Abramov,
Megan A. Johnson,
Eve-Ellen Govek,
Jacqueline Burré,
Teresa A. Milner,
Natalia V. De Marco García
Affiliations
Andrew F. Iannone
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA; Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, NY 10021, USA
Gülcan Akgül
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Robin Zhang
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Sam Wacks
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Nisma Hussein
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Carmen Ginelly Macias
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Alexander Donatelle
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Julia M.J. Bauriedel
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Cora Wright
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Debra Abramov
Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, NY 10021, USA; Appel Alzheimer’s Disease Research Institute, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Megan A. Johnson
Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Eve-Ellen Govek
Laboratory of Developmental Neurobiology, The Rockefeller University, New York, NY 10065, USA
Jacqueline Burré
Appel Alzheimer’s Disease Research Institute, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Teresa A. Milner
Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA
Natalia V. De Marco García
Center for Neurogenetics, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10021, USA; Corresponding author
Summary: Spontaneous and sensory-evoked activity sculpts developing circuits. Yet, how these activity patterns intersect with cellular programs regulating the differentiation of neuronal subtypes is not well understood. Through electrophysiological and in vivo longitudinal analyses, we show that C-X-C motif chemokine ligand 14 (Cxcl14), a gene previously characterized for its association with tumor invasion, is expressed by single-bouquet cells (SBCs) in layer I (LI) of the somatosensory cortex during development. Sensory deprivation at neonatal stages markedly decreases Cxcl14 expression. Additionally, we report that loss of function of this gene leads to increased intrinsic excitability of SBCs—but not LI neurogliaform cells—and augments neuronal complexity. Furthermore, Cxcl14 loss impairs sensory map formation and compromises the in vivo recruitment of superficial interneurons by sensory inputs. These results indicate that Cxcl14 is required for LI differentiation and demonstrate the emergent role of chemokines as key players in cortical network development.
