THE DAMAGE OF MEMBRANES STRUCTURE OF HEPATOCYTES IN RATS DURING FLUORIDE INTOXICATION

Abstract

The study was performed at the Department of Clinical Pathophysiology, Topographical Anatomy and Operative Surgery at Kharkiv Medical Academy of Postgraduate Education according to the research project “Radiotoxins' pathochemical mechanisms on the body and methods of early diagnostics and correction” 0117U000589. We studied the effect of small subtoxic doses of sodium fluoride on the phospholipid composition of hepatocyte membranes of 30 rats of the Wistar line in the subacute experiment. The analysis of the obtained results allows us to confirm the disrution of the quantitative content of general and individual phospholipids in lipid extracts of hepatocyte membranes, depending on the dose and duration of xenobiotic activity. The calculation of the ratio of the amount of rapidly oxidizing phospholipid fractions (phosphatidylserine, phosphatidyl ethanolamine, phosphatidylinositol) to the sum of severely oxidizing phospholipid fractions (sphingomyelin, phosphatidylcholine, lysophosphatidylcholine) with sodium fluoride at a dose of 1/10 LD50 indicates a decrease by 1,4 and 2,3 times respectively on the 30th and 60th day of the experiment, reflecting the depletion of the adaptive capacity of the liver cells. In case of sodium fluoride at a dose of 1/100 LD50, this factor increases by 1.2 times on the 30th day of the experiment, and on the 60th day it decreases by 1.6 times, which reflects the stresses of the adaptive potential with its subsequent breakdown.

Keywords

• sodium fluoride, phospholipid composition of hepatocyte membranes, phospholipid fractions, rats of wistar line