Construction of complete degradation pathway for nitrobenzene in Escherichia coli

Yong-Dong Deng; Li-Juan Wang; Wen-Hui Zhang; Jing Xu; Jian-Jie Gao; Bo Wang; Xiao-Yan Fu; Hong-Juan Han; Zhen-Jun Li; Yu Wang; Yong-Sheng Tian; Ri-He Peng; Quan-Hong Yao

Ecotoxicology and Environmental Safety (Sep 2022)

Construction of complete degradation pathway for nitrobenzene in Escherichia coli

Yong-Dong Deng,
Li-Juan Wang,
Wen-Hui Zhang,
Jing Xu,
Jian-Jie Gao,
Bo Wang,
Xiao-Yan Fu,
Hong-Juan Han,
Zhen-Jun Li,
Yu Wang,
Yong-Sheng Tian,
Ri-He Peng,
Quan-Hong Yao

Affiliations

Yong-Dong Deng: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Li-Juan Wang: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Wen-Hui Zhang: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Jing Xu: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Jian-Jie Gao: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Bo Wang: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Xiao-Yan Fu: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Hong-Juan Han: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Zhen-Jun Li: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Yu Wang: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China
Yong-Sheng Tian: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China; Corresponding authors at: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai, China.
Ri-He Peng: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China; Corresponding authors at: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai, China.
Quan-Hong Yao: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901, Beidi Road, Shanghai, China; Key Laboratory for Safety Assessment (Enviornment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Shanghai, China; Corresponding authors at: Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai, China.

Journal volume & issue: Vol. 243
p. 114016

Abstract

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Nitrobenzene is widely present in industrial wastewater and soil. Biodegradation has become an ideal method to remediate organic pollutants due to its low cost, high efficiency, and absence of secondary pollution. In the present study, 10 exogenous genes that can completely degrade nitrobenzene were introduced into Escherichia coli, and their successful expression in the strain was verified by fluorescence quantitative polymerase chain reaction and proteomic analysis. The results of the degradation experiment showed that the engineered strain could completely degrade 4 mM nitrobenzene within 8 h. The formation of intermediate metabolites was detected, and the final metabolites entered the E. coli tricarboxylic acid cycle smoothly. This process was discovered by isotope tracing method. Results indicated the integrality of the degradation pathway and the complete degradation of nitrobenzene. Finally, further experiments were conducted in soil to verify its degradation ability and showed that the engineered strain could also degrade 1 mM nitrobenzene within 10 h. In this study, engineered bacteria that can completely degrade nitrobenzene have been constructed successfully. The construction of remediation-engineered bacteria by synthetic biology laid the foundation for the industrial application of biological degradation of organic pollutants.

Published in Ecotoxicology and Environmental Safety

ISSN: 0147-6513 (Print); 1090-2414 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Environmental technology. Sanitary engineering: Environmental pollution; Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/ecotoxicology-and-environmental-safety

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