Increased expression of m6A demethylase FTO inhibits the proliferation of nasopharyngeal carcinoma cells

Abstract

Objective To investigate the expression of N6 methyladenine (m6A) demethylase human fat mass and obesity-associated(FTO) protein in nasopharyngeal carcinoma (NPC), and the effect of over-expression of FTO on the proliferation of nasopharyngeal carcinoma in vitro and in vivo. Methods Immunohistochemistry method was used to detect the expression of FTO protein in nasopharyngeal carcinoma tissues and para-cancerous tissues; The dominant expression cell line of FTO was screened, the over-expression FTO cell line was constructed. The cell proliferation was examined by soft-agar method. A mouse tumor model was developed for measurement of tumor growth. Results The expression of FTO in nasopharyngeal carcinoma tissues was lower than that in adjacent tissues. Low expression of FTO promoted proliferation of NPC cells, while over-expression of FTO reversed this effect. Conclusions FTO inhibits proliferation of nasopharyngeal carcinoma and this result may provide an experimental technology in searching therapeutic targets of chemotherapy for nasopharyngeal carcinoma.

Keywords

• nasopharyngeal carcinoma|n6-methyladenosine|human fat mass and obesity-associated proteins|cell proliferation