Integration of Genotoxic Biomarkers in Environmental Biomonitoring Analysis Using a Multi-Biomarker Approach in Three-Spined Stickleback (<i>Gasterosteus aculeatus</i> Linnaeus, 1758)
Amélie Cant,
Marc Bonnard,
Jean-Marc Porcher,
Jean Prygiel,
Audrey Catteau,
Laurence Delahaut,
Olivier Palluel,
Cyril Turiès,
Alain Geffard,
Anne Bado-Nilles
Affiliations
Amélie Cant
Institut National de l’Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France
Marc Bonnard
Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, UFR Sciences Exactes et Naturelles, Campus Moulin de la Housse, BP 1039, 51687 Reims, France
Jean-Marc Porcher
Institut National de l’Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France
Jean Prygiel
Agence de l’Eau Artois-Picardie, 200 Rue Marceline—Centre Tertiaire de l’Arsenal, BP 80818, 59508 Douai, France
Audrey Catteau
Institut National de l’Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France
Laurence Delahaut
Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, UFR Sciences Exactes et Naturelles, Campus Moulin de la Housse, BP 1039, 51687 Reims, France
Olivier Palluel
Institut National de l’Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France
Cyril Turiès
Institut National de l’Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France
Alain Geffard
Université de Reims Champagne-Ardenne (URCA), UMR-I 02 SEBIO, UFR Sciences Exactes et Naturelles, Campus Moulin de la Housse, BP 1039, 51687 Reims, France
Anne Bado-Nilles
Institut National de l’Environnement Industriel et des Risques (INERIS), UMR-I 02 SEBIO, Parc Technologique Alata, BP 2, 60550 Verneuil-en-Halatte, France
Water is impacted by a variety of increasing pressures, such as contaminants, including genotoxic pollutants. The proposed multi-biomarker approach at a sub-individual level gives a complementary indicator to the chemical and ecological parameters of the Water Framework Directive (WFD, 2000/60/EC). By integrating biomarkers of genotoxicity and erythrocyte necrosis in the sentinel fish species the three-spined stickleback (Gasterosteus aculeatus) through active biomonitoring of six stations of the Artois-Picardie watershed, north France, our work aimed to improve the already existing biomarker approach. Even if fish in all stations had high levels of DNA strand breaks, the multivariate analysis (PCA), followed by hierarchical agglomerative clustering (HAC), improved discrimination among stations by detecting an increase of nuclear DNA content variation (Etaing, St Rémy du Nord, Artres and Biache-St-Vaast) and erythrocyte necrosis (Etaing, St Rémy du Nord). The present work highlighted that the integration of these biomarkers of genotoxicity in a multi-biomarker approach is appropriate to expand physiological parameters which allow the targeting of new potential effects of contaminants.
