Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery

Wen Song; Qingyuan Ye; Zhe Li; Yajie Hou; Lina Niu; Zhiwei Ma; Chuanxu Yang

Materials & Design (Jul 2022)

Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery

Wen Song,
Qingyuan Ye,
Zhe Li,
Yajie Hou,
Lina Niu,
Zhiwei Ma,
Chuanxu Yang

Affiliations

Wen Song: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.
Qingyuan Ye: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China
Zhe Li: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China
Yajie Hou: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China
Lina Niu: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China
Zhiwei Ma: State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.
Chuanxu Yang: Key Laboratory Colloid and Interface Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; Corresponding authors.

Journal volume & issue: Vol. 219
p. 110748

Abstract

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It is well known that the chitosan (CS) properties such as molecular weight (Mw) and deacetylation degree (DD) are crucial for siRNA delivery. In the present study, we have unintentionally found that the transfection medium has much more substantial effect, which has not been reported before. Specifically, the transfection efficiency was considerably increased in Hank’s Balanced Saline Solution (HBSS), with various Mw (30–150 kD) and DD (85–95%) combinations in different cell types. In addition, even though the CS/siRNA nanoparticles physical parameters such as diameter size and polydisperse index were disturbed by cell culture medium or serum, a relatively high knockdown efficiency could still be obtained in HBSS. Moreover, the highly efficient silencing in HBSS even did not require pre-mixing to formulate the CS/siRNA nanoparticles. In conclusion, our findings have confirmed a neglected but crucial important factor of transfection medium in the chitosan mediated siRNA delivery.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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