Revista Ciencias de la Salud (Dec 2008)
Primary culture of human keratinocytes planted on pig’s intestine
Abstract
Tissue engineering, in the fields of skin regeneration,seeks to overcome the limitationsassociated with the use of immediate auto-grafts,since choosing the donor region of the patientconstitutes a risk for the patient himself and isinsufficient if the injury that has to be repairedis very large. The use of the pig’s small intestinesubmucosa (SIS) has being proved as a fillingbiomaterial to treat injuries, because of its specialcomposition, it lowers pain and inflammationsince its first application and it favours earlymobility to the wounded area. The present studydeveloped a protocol for the primary cultureof human keratinocytes from infant foreskins,and used small intestinal submucosa (SIS) likeculture substrate. Cellular adherence potentialand proliferation capability of the keratinocytesover this substrate was evaluated.