Analysis of the binary mixtures of amlodipine and atorvastatin by chemometric-spectophotometric method

Abstract

In this study, the simultaneous quantification of amlodipine and atorvastatin in pharmaceutical preparations was successfully accomplished without the need for a separation process by using chemometric calibration methods (principal component analysis method (PCA), principal component regression method (PCR), and partial least squares method (PLS). Additionally, chemometric evaluation was performed on the data obtained from UV visible field spectroscopy methods. 16 mixes containing the chemicals atorvastatin and amlodipine were created as a calibration (concentration) set to assess the validity of the designated chemometric procedures. The calibration set's absorption spectrum was captured between 200 and 500 nm. The link between the calibration set and the absorption data collected in the 215–355 nm region was used to produce three chemometric calibrations. Amlodipine and atorvastatin synthetic mixtures were analyzed to assess the validity of the PCA, PCR, and PLS approaches. The percentage (%) recovery average for atorvastatin and amlodipine was determined for both the drug sample and the synthetic mixture using the approach we used. It was discovered that the computed values were fairly high.The methodologies were controlled, and the ANOVA test was run using the results of the PLS and PCR calibration techniques. The enhanced technique might be suggested for the examination of medication samples containing atorvastatin and amlodipine since it is among the most sensitive, accurate, and repeatable techniques available.

Keywords

• amlodipine
• atorvastatin
• chemometry