Sustainable Effects of Human Dental Pulp Stem Cell Transplantation on Diabetic Polyneuropathy in Streptozotocine-Induced Type 1 Diabetes Model Mice

Masaki Hata; Maiko Omi; Yasuko Kobayashi; Nobuhisa Nakamura; Megumi Miyabe; Mizuho Ito; Tasuku Ohno; Yuka Imanishi; Tatsuhito Himeno; Hideki Kamiya; Jiro Nakamura; Hitoshi Miyachi; Shogo Ozawa; Ken Miyazawa; Akio Mitani; Toru Nagao; Shigemi Goto; Jun Takebe; Tatsuaki Matsubara; Keiko Naruse

doi:10.3390/cells10092473

Cells (Sep 2021)

Sustainable Effects of Human Dental Pulp Stem Cell Transplantation on Diabetic Polyneuropathy in Streptozotocine-Induced Type 1 Diabetes Model Mice

Masaki Hata,
Maiko Omi,
Yasuko Kobayashi,
Nobuhisa Nakamura,
Megumi Miyabe,
Mizuho Ito,
Tasuku Ohno,
Yuka Imanishi,
Tatsuhito Himeno,
Hideki Kamiya,
Jiro Nakamura,
Hitoshi Miyachi,
Shogo Ozawa,
Ken Miyazawa,
Akio Mitani,
Toru Nagao,
Shigemi Goto,
Jun Takebe,
Tatsuaki Matsubara,
Keiko Naruse

Affiliations

Masaki Hata: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Maiko Omi: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Yasuko Kobayashi: Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Nobuhisa Nakamura: Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Megumi Miyabe: Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Mizuho Ito: Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Tasuku Ohno: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Yuka Imanishi: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Tatsuhito Himeno: Division of Diabetes, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan
Hideki Kamiya: Division of Diabetes, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan
Jiro Nakamura: Division of Diabetes, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan
Hitoshi Miyachi: Department of Maxillofacial Surgery, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Shogo Ozawa: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Ken Miyazawa: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Akio Mitani: Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Toru Nagao: Department of Maxillofacial Surgery, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Shigemi Goto: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Jun Takebe: Department of Removable Prosthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Tatsuaki Matsubara: Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan
Keiko Naruse: Department of Internal Medicine, School of Dentistry, Aichi Gakuin University, Nagoya 464-8651, Japan

DOI: https://doi.org/10.3390/cells10092473
Journal volume & issue: Vol. 10, no. 9
p. 2473

Abstract

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Dental pulp stem cells (DPSCs) are suitable for use in regenerative medicine. Cryopreserved human DPSCs (hDPSCs) ameliorate diabetic polyneuropathy, and the effects of hDPSC transplantation are related to VEGF and NGF secretion. This study evaluated the long-term effects of a single transplantation of hDPSCs on diabetic polyneuropathy. hDPSCs were obtained from human third molars extracted for orthodontic treatment, which were then transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocin injection in nude mice. The effects of hDPSC transplantation were analyzed at 16 weeks post-transplantation. DPSC transplantation significantly improved delayed nerve conduction velocity, decreased blood flow, and increased sensory perception thresholds. Furthermore, the hDPSC-conditioned medium promoted the neurite outgrowth of dorsal root ganglion neurons. In conclusion, the therapeutic effects of hDPSC transplantation with a single injection last for prolonged periods and may be beneficial in treating long-term diabetic polyneuropathy.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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