Physiological and Biochemical Parameters of Common Duckweed <i>Lemna minor</i> after the Exposure to Tetracycline and the Recovery from This Stress
Magdalena Krupka,
Dariusz J. Michalczyk,
Jūratė Žaltauskaitė,
Gintarė Sujetovienė,
Katarzyna Głowacka,
Hanna Grajek,
Marta Wierzbicka,
Agnieszka I. Piotrowicz-Cieślak
Affiliations
Magdalena Krupka
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland
Dariusz J. Michalczyk
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland
Jūratė Žaltauskaitė
Department of Environmental Sciences, Vytautas Magnus University, Universiteto 10, 46265 Kaunas, Lithuania
Gintarė Sujetovienė
Department of Environmental Sciences, Vytautas Magnus University, Universiteto 10, 46265 Kaunas, Lithuania
Katarzyna Głowacka
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland
Hanna Grajek
Department of Physics and Biophysics, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland
Marta Wierzbicka
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland
Agnieszka I. Piotrowicz-Cieślak
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-718 Olsztyn, Poland
In this study, the ability of Lemna minor L. to recover to normal growth, after being degraded in a tetracycline-containing medium, was extensively investigated. The plants were exposed to tetracycline (TC) at concentrations of 1, 2.5, and 10 mM. Subsequently, their physiological status was analysed against the following criteria: rate of plant growth; free radical accumulation; antioxidant enzyme activity; chlorophyll content; HSP70 protein content; cell membrane permeability, and mitochondrial activity. The study showed that duckweed can considerably recover from the damage caused by antibiotics, within a week of cessation of stress. Of the plant properties analysed, mitochondrial activity was the most sensitive to antibiotic-induced disturbances. After transferring the plants to a tetracycline-free medium, all plant parameters improved significantly, except for the mitochondrial activity in the plants grown on the medium containing the highest dose of tetracycline. In the plants treated with this antibiotic at the concentration of 10 mM, the proportion of dead mitochondria increased and was as high as 93% after one week from the beginning of the recovery phase, even after the transfer to the tetracycline-free medium.
