Acta Ortopédica Brasileira (Jan 2014)

Laser therapy in bone repair in rats: analysis of bone optical density

  • Danillo Barbosa,
  • Antonio Guillermo Jose Balbin Villaverde,
  • Emilia Ângela LoschiavoArisawa,
  • Renato Aparecido de Souza

DOI
https://doi.org/10.1590/1413-78522014220200438
Journal volume & issue
Vol. 22, no. 2
pp. 71 – 74

Abstract

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OBJECTIVE: To investigate, by digital radiology, the bone regeneration process in rats submitted to femoral osteotomy and treated with low power laser therapy.METHODS: Forty-five Wistar rats were subjected to transverse osteotomy of the right femur and divided randomly into three experimental groups (n = 15): animals not treated with laser therapy G (C), animals that received laser therapy with λ: 660nm G (660nm) and animals that received laser therapy with λ: 830nm G (830nm). Animals were sacrificed after 7, 14 and 21 days. The bone calluses were evaluated by digital X-ray at 65 kVp, 7mA and 0.032 s exposures.RESULTS: The values obtained were submitted to variance analysis (ANOVA) followed by the Tukey-Kramer test. The significance level adopted was 5%. The groups G (C), G (660nm), and G (830nm) at the 7th day showed a significant bone development, with p <0.0116; the groups G (C), G (660nm), and G (830nm) at the 14th day showed values of p <0.0001; at the 21stday,a higher degree of bone repair were observed in group G (830nm), and G (660nm), with p <0.0169.CONCLUSION: Based on the radiographic findings, G (830nm) showed more complete bone regeneration, as shown in the gray shades of the images. Level of Evidence II, Individual Study With Experimental Design.

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