Nature Communications (Sep 2020)

Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing

  • Minh Thuan Nguyen Tran,
  • Mohd Khairul Nizam Mohd Khalid,
  • Qi Wang,
  • Jacqueline K. R. Walker,
  • Grace E. Lidgerwood,
  • Kimberley L. Dilworth,
  • Leszek Lisowski,
  • Alice Pébay,
  • Alex W. Hewitt

DOI
https://doi.org/10.1038/s41467-020-18715-y
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 10

Abstract

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Off-target effects and the feasibility for AAV-mediated delivery are the major barriers impeding the clinical in vivo application of base editors. Here, the authors report the small size AAV-deliverable Cas9-ABE variant that has improved on-target editing efficiency and reduced RNA-off target footprint.