The analysis of the circRNAs in the progress of acquired resistance to Cetuximab

Abstract

Abstract We established a Cetuximab‐resistant cell lines by high‐dose pulse method and searched for differentially expressed circular RNAs by RNA sequencing. Hundreds of circRNAs were altered between sensitive and resistant cells. Next, we chose six notably differential circRNAs and conducted quantitative real‐time PCR by specific primers. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that differentially expressed circular RNAs are enriched in some tumor‐related pathways, such as tumor transcription regulation, metabolism, PI3K‐Akt, mTOR, and other signaling pathways. Our results explored differentially expressed circular RNAs associated with Cetuximab to find new targets for Cetuximab resistance therapy.

Keywords

• Cetuximab resistance
• circRNA
• colorectal cancer
• RNA‐seq