Journal of Enzyme Inhibition and Medicinal Chemistry (Jan 2018)

Determination and diagnostic value of CA9 mRNA in peripheral blood of patients with oral leukoplakia

  • Manuel Torres López,
  • Mario Pérez Sayáns,
  • Cintia Chamorro Petronacci,
  • Francisco Barros Angueira,
  • Pilar Gándara Vila,
  • Alejandro Lorenzo Pouso,
  • Abel García García

DOI
https://doi.org/10.1080/14756366.2018.1466120
Journal volume & issue
Vol. 33, no. 1
pp. 951 – 955

Abstract

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Background: Oral leukoplakia is one of the most common oral premalignant disorder. The classical evaluation through tissue biopsy is not always valid to evaluate the risk of malignization. Material and methods: RT-qPCR was performed on 47 blood samples (21 patients with leukoplakia, 2 with oral squamous cell carcinoma (OSCC), and 24 healthy patients) and on 11 tissue samples (3 leukoplakia, 4 OSCC, and 4 samples of healthy tissue). Results: There are significant differences in expression between the different groups (F = 4.057, p = .006). The Duncan post hoc test shows that the only group that differentiates is the tumour tissue. Using Wilcoxon test, different covariables of patients with leukoplakia were analysed with respect to the group of healthy patients and no significant differences were observed. Conclusions: The diagnostic route through liquid biopsy has not been conclusive in this study, but there are significant differences in the levels analysed in the different tissue samples.

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