An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients

Anne C. Gilmore; Sarah J. Flaherty; Veena Somasundaram; David A. Scheiblin; Stephen J. Lockett; David A. Wink; William F. Heinz

doi:10.1038/s42003-021-01954-0

Communications Biology (Apr 2021)

An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients

Anne C. Gilmore,
Sarah J. Flaherty,
Veena Somasundaram,
David A. Scheiblin,
Stephen J. Lockett,
David A. Wink,
William F. Heinz

Affiliations

Anne C. Gilmore: Optical Microscopy and Analysis Laboratory, Office of Science and Technology Resources, Center for Cancer Research, National Cancer Institute, National Institutes of Health
Sarah J. Flaherty: Optical Microscopy and Analysis Laboratory, Office of Science and Technology Resources, Center for Cancer Research, National Cancer Institute, National Institutes of Health
Veena Somasundaram: Laboratory of Cancer Immunometabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health
David A. Scheiblin: Optical Microscopy and Analysis Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research
Stephen J. Lockett: Optical Microscopy and Analysis Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research
David A. Wink: Laboratory of Cancer Immunometabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health
William F. Heinz: Optical Microscopy and Analysis Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

DOI: https://doi.org/10.1038/s42003-021-01954-0
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 13

Abstract

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Gilmore et al. describe an in vitro tumorigenesis model that produces cell-generated gradients of oxygen and nutrients. Its 2D configuration simplifies live cell, immunofluorescent, and microscopic interrogation of cancer cell behaviour in differentially developed tumour microenvironment conditions, including co-culture systems.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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