Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

Duy P. Nguyen; Yuichiro Miyaoka; Luke A. Gilbert; Steven J. Mayerl; Brian H. Lee; Jonathan S. Weissman; Bruce R. Conklin; James A. Wells

doi:10.1038/ncomms12009

Nature Communications (Jul 2016)

Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

Duy P. Nguyen,
Yuichiro Miyaoka,
Luke A. Gilbert,
Steven J. Mayerl,
Brian H. Lee,
Jonathan S. Weissman,
Bruce R. Conklin,
James A. Wells

Affiliations

Duy P. Nguyen: Department of Pharmaceutical Chemistry, University of California, San Francisco
Yuichiro Miyaoka: Gladstone Institute of Cardiovascular Disease
Luke A. Gilbert: Department of Cellular and Molecular Pharmacology, University of California, San Francisco
Steven J. Mayerl: Gladstone Institute of Cardiovascular Disease
Brian H. Lee: Department of Pharmaceutical Chemistry, University of California, San Francisco
Jonathan S. Weissman: Department of Cellular and Molecular Pharmacology, University of California, San Francisco
Bruce R. Conklin: Gladstone Institute of Cardiovascular Disease
James A. Wells: Department of Pharmaceutical Chemistry, University of California, San Francisco

DOI: https://doi.org/10.1038/ncomms12009
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

Read online

CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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