Validation of HPLC Method for the Determination of 5-hydroxymethylfurfural in Pestil, Köme, Jam, Marmalade and Pekmez

Abstract

This study represents a high performance liquid chromatography HPLC method for the detection of 5-hydroxymethylfurfural in pestil, köme, jam and pekmez samples. The linearity, selectivity, decision limit, detection capability, detection limit, quantification limit, precision, recovery, ruggedness and measurement uncertainty of the method were determined. The developed method, simple and accurate, showed good recovery values 97-108% . The accuracy of the method expressed with the relative standard deviation was below 6%. The detection limit and quantification limit were 0.03 mg/kg and 0.10 mg/kg, respectively. HMF levels in pestil, köme, jam, marmalade and pekmez samples were determined using the validated method

Keywords

• hmf
• method validation
• pestil
• köme
• jam
• marmalade and pekmez.