Genetic Characteristics of Multiple Copies of Tn1546-Like Elements in ermB-Positive Methicillin-Resistant Staphylococcus aureus From Mainland China

Haiping Wang; Haiping Wang; Haiping Wang; Dandan Wu; Lingfang Di; Feiteng Zhu; Feiteng Zhu; Feiteng Zhu; Zhengan Wang; Zhengan Wang; Zhengan Wang; Lu Sun; Lu Sun; Lu Sun; Yiyi Chen; Yiyi Chen; Yiyi Chen; Shengnan Jiang; Shengnan Jiang; Shengnan Jiang; Hemu Zhuang; Hemu Zhuang; Hemu Zhuang; Mengzhen Chen; Mengzhen Chen; Mengzhen Chen; Shujuan Ji; Shujuan Ji; Shujuan Ji; Yan Chen; Yan Chen; Yan Chen; Yan Chen

doi:10.3389/fmicb.2022.814062

Frontiers in Microbiology (Feb 2022)

Genetic Characteristics of Multiple Copies of Tn1546-Like Elements in ermB-Positive Methicillin-Resistant Staphylococcus aureus From Mainland China

Haiping Wang,
Haiping Wang,
Haiping Wang,
Dandan Wu,
Lingfang Di,
Feiteng Zhu,
Feiteng Zhu,
Feiteng Zhu,
Zhengan Wang,
Zhengan Wang,
Zhengan Wang,
Lu Sun,
Lu Sun,
Lu Sun,
Yiyi Chen,
Yiyi Chen,
Yiyi Chen,
Shengnan Jiang,
Shengnan Jiang,
Shengnan Jiang,
Hemu Zhuang,
Hemu Zhuang,
Hemu Zhuang,
Mengzhen Chen,
Mengzhen Chen,
Mengzhen Chen,
Shujuan Ji,
Shujuan Ji,
Shujuan Ji,
Yan Chen,
Yan Chen,
Yan Chen,
Yan Chen

Affiliations

Haiping Wang: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Haiping Wang: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Haiping Wang: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Dandan Wu: Department of Infectious Diseases, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lingfang Di: Department of Clinical Laboratory, Tongxiang First people’s hospital, Tongxiang, China
Feiteng Zhu: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Feiteng Zhu: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Feiteng Zhu: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Zhengan Wang: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Zhengan Wang: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Zhengan Wang: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lu Sun: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Lu Sun: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Lu Sun: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yiyi Chen: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yiyi Chen: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Yiyi Chen: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Shengnan Jiang: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Shengnan Jiang: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Shengnan Jiang: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Hemu Zhuang: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Hemu Zhuang: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Hemu Zhuang: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Mengzhen Chen: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Mengzhen Chen: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Mengzhen Chen: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Shujuan Ji: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Shujuan Ji: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Shujuan Ji: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yan Chen: Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yan Chen: Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, China
Yan Chen: Regional Medical Center for National Institute of Respiratory Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
Yan Chen: Department of Hospital Epidemiology and Infection Control, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China

DOI: https://doi.org/10.3389/fmicb.2022.814062
Journal volume & issue: Vol. 13

Abstract

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ObjectiveTo determine the genetic structure of ermB-positive Tn1546-like mobile elements in methicillin-resistant Staphylococcus aureus (MRSA) from mainland China.MethodsA total of 271 erythromycin-resistant MRSA isolates were isolated from Sir Run Run Shaw Hospital (SRRSH) from 2013 to 2015. Whole-genome sequencing was performed for the ermB-positive strains, and the genetic environment of the ermB genes was analyzed. Southern hybridization analysis and transformation tests were performed to confirm the location of the ermB gene.ResultsA total of 64 isolates (64/271, 23.6%) were ermB-positive strains, with 62 strains (62/64, 96.9%) belonging to the CC59 clone. The other two strains, SR130 and SR231, belonging to CC5-ST965, both harbored 14,567 bp ermB-positive Tn1546-like elements and displayed multidrug-resistant profiles. PFGE followed by Southern blot demonstrated that the ermB genes were located on the plasmids of both SR130 and SR231, while two copies of ermB were located on the chromosome of SR231. Further sequencing demonstrated that SR231 carried one Tn1546-ermB elements in the plasmid and two identical copies integrated on the chromosome, which had 99.99% identity to the element in the plasmid of SR130. The Tn1546-ermB elements were highly similar (100% coverage, >99.9% identity) to the element Tn6636 reported in a previous study from Taiwan. The plasmids (pSR130 and pSR231) harboring ermB-positive Tn1546-like elements were also identical to the mosaic plasmid pNTUH_5066148. However, conjugation of ermB-carrying plasmids of SR130 and SR231 were failed after triple repeats.ConclusionMultiple copies of ermB-positive Tn1546-like mobile elements were found in CC5-ST965 MRSA from mainland China, showing the wide dissemination of these Enterococcus faecium-originated ermB-positive Tn1546-like elements. Molecular epidemiological study of Tn1546-like elements is essential to avoid the spreading of resistant determinants.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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