Synthesis of biogenic silver nanoparticles using butter fruit pulp extract and evaluation of their antibacterial activity against Providencia vermicola in Rohu

Abstract

Objective: Development of antimicrobial materials using nano approach and several industries like aquaculture greatly depend on novel biogenic materials. Biogenic techniques to develop nanomaterials with potent antimicrobial activity have been explored recently. The present study demonstrates the green synthesis of AgNPs using butter fruit (Persea americana) pulp extract and its antibacterial efficacy against the fish pathogen Providencia vermicola using Rohu fish. Results: The AgNPs were prepared and characterized using various spectroscopic and microscopic techniques. The infrared spectroscopic analysis identified that the fruit biological molecules were involved in the stabilization of AgNPs. Transmission electron microscopic analysis revealed that particles size ranged from 20 to 50 nm. Further, the nanoparticles (5 µg) encapsulated fish feed were given to P. vermicola infected Rohu fish. The survival rate observed was 72 % in experimental group as compared to the control group. Total plate count and histopathological results indicated that the AgNPs treated groups showed significant reduction of bacterial population and restore the tissues in the normal range. Conclusion: The results suggest that the green synthesized (AgNPs) using butter fruit pulp have good efficiency in reducing the infection caused by P. vermicola in Rohu fish.

Keywords

• Silver nanoparticles
• Persea americana
• Labeo rohita
• Spectroscopy
• Histopathology: Antibacterial activity