Frontiers in Immunology (May 2024)
Dietary 5-hydroxytryptophan improves sheep growth performance by enhancing ruminal functions, antioxidant capacity, and tryptophan metabolism: in vitro and in vivo studies
- Zhe Sun,
- Zhe Sun,
- Zhe Sun,
- Zhe Sun,
- Natnael D. Aschalew,
- Natnael D. Aschalew,
- Natnael D. Aschalew,
- Long Cheng,
- Long Cheng,
- Yuanhong Xia,
- Yuanhong Xia,
- Longyu Zhang,
- Longyu Zhang,
- Guopei Yin,
- Guopei Yin,
- Shikun Wang,
- Ziyuan Wang,
- Ziyuan Wang,
- Jianan Dong,
- Jianan Dong,
- Weigang Zhang,
- Wei Zhao,
- Guixin Qin,
- Guixin Qin,
- Xuefeng Zhang,
- Xuefeng Zhang,
- Rongzhen Zhong,
- Tao Wang,
- Tao Wang,
- Tao Wang,
- Yuguo Zhen,
- Yuguo Zhen,
- Yuguo Zhen
Affiliations
- Zhe Sun
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Zhe Sun
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Zhe Sun
- College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, China
- Zhe Sun
- Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China
- Natnael D. Aschalew
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Natnael D. Aschalew
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Natnael D. Aschalew
- College of Agriculture and Environmental Science, Dilla University, Dilla, Ethiopia
- Long Cheng
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Long Cheng
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Yuanhong Xia
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Yuanhong Xia
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Longyu Zhang
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Longyu Zhang
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Guopei Yin
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Guopei Yin
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Shikun Wang
- College of Life Sciences, Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, China
- Ziyuan Wang
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Ziyuan Wang
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Jianan Dong
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Jianan Dong
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Weigang Zhang
- Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China
- Wei Zhao
- Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China
- Guixin Qin
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Guixin Qin
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Xuefeng Zhang
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Xuefeng Zhang
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Rongzhen Zhong
- Jilin Province Feed Processing and Ruminant Precision Breeding Cross-Regional Cooperation Technology Innovation Center, Jilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China
- Tao Wang
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Tao Wang
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Tao Wang
- Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China
- Yuguo Zhen
- Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Yuguo Zhen
- Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China
- Yuguo Zhen
- Postdoctoral Scientific Research Workstation, Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Science & Technology Co. Ltd., Changchun, China
- DOI
- https://doi.org/10.3389/fimmu.2024.1398310
- Journal volume & issue
-
Vol. 15
Abstract
BackgroundHydroxytryptophan (5-HTP) can regulate the synthesis of 5-Hydroxytryptamine (5-HT) and melatonin (MT). In a previous metabolome analysis, we found that 5-HTP is an effective ingredient in yeast culture for regulating rumen fermentation. However, research on the effect of this microbial product (5-HTP) as a functional feed additive in sheep production is still not well explained. Therefore, this study examined the effects of 5-HTP on sheep rumen function and growth performance using in vitro and in vivo models.MethodsA two-factor in vitro experiment involving different 5-HTP doses and fermentation times was conducted. Then, in the in vivo experiment, 10 sheep were divided into a control group which was fed a basal diet, and a 5-HTP group supplemented with 8 mg/kg 5-HTP for 60 days.ResultsThe results showed that 5-HTP supplementation had a significant effect on in vitro DMD, pH, NH3-N, acetic acid, propionic acid, and TVFA concentrations. 5-HTP altered rumen bacteria composition and diversity indices including Chao1, Shannon, and Simpson. Moreover, the in vivo study on sheep confirmed that supplementing with 8 mg/kg of 5-HTP improved rumen fermentation efficiency and microbial composition. This led to enhanced sheep growth performance and increased involvement in the tryptophan metabolic pathway, suggesting potential benefits.ConclusionDietary 5-HTP (8 mg/kg DM) improves sheep growth performance by enhancing ruminal functions, antioxidant capacity, and tryptophan metabolism. This study can provide a foundation for the development of 5-HTP as a functional feed additive in ruminants’ production.
Keywords
