Detection of Influenza Virus Using a SOI-Nanoribbon Chip, Based on an N-Type Field-Effect Transistor
Kristina A. Malsagova,
Tatyana O. Pleshakova,
Andrey F. Kozlov,
Rafael A. Galiullin,
Vladimir P. Popov,
Fedor V. Tikhonenko,
Alexander V. Glukhov,
Vadim S. Ziborov,
Ivan D. Shumov,
Oleg F. Petrov,
Vladimir M. Generalov,
Anastasia A. Cheremiskina,
Alexander G. Durumanov,
Alexander P. Agafonov,
Elena V. Gavrilova,
Rinat A. Maksyutov,
Alexander S. Safatov,
Valentin G. Nikitaev,
Alexander N. Pronichev,
Vladimir A. Konev,
Alexander I. Archakov,
Yuri D. Ivanov
Affiliations
Kristina A. Malsagova
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Tatyana O. Pleshakova
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Andrey F. Kozlov
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Rafael A. Galiullin
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Vladimir P. Popov
Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia
Fedor V. Tikhonenko
Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia
Alexander V. Glukhov
JSC Novosibirsk Plant of Semiconductor Devices with OKB, 630082 Novosibirsk, Russia
Vadim S. Ziborov
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Ivan D. Shumov
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Oleg F. Petrov
Joint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, Russia
Vladimir M. Generalov
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Anastasia A. Cheremiskina
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Alexander G. Durumanov
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Alexander P. Agafonov
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Elena V. Gavrilova
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Rinat A. Maksyutov
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Alexander S. Safatov
Federal Budgetary Research Institution—State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance on Consumer Rights Protection and Human Well-Being, 630559 Novosibirsk Region, Koltsovo, Russia
Valentin G. Nikitaev
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russia
Alexander N. Pronichev
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, Russia
Vladimir A. Konev
Department of Infectious Diseases in Children, Faculty of Pediatrics, Pirogov Russian National Research Medical University, 117997 Moscow, Russia
Alexander I. Archakov
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
Yuri D. Ivanov
Laboratory of Nanobiotechnology, Institute of Biomedical Chemistry, 119121 Moscow, Russia
The detection of influenza A virions with a nanoribbon detector (NR detector) has been demonstrated. Chips for the detector have been fabricated based on silicon-on-insulator nanoribbon structures (SOI nanoribbon chip), using a complementary metal-oxide-semiconductor (CMOS)-compatible technology—by means of gas-phase etching and standard optical photolithography. The surface of the SOI nanoribbon chip contains a matrix of 10 nanoribbon (NR) sensor elements. SOI nanoribbon chips of n-type conductance have been used for this study. For biospecific detection of target particles, antibodies against influenza virus have been covalently immobilized onto NRs. Influenza A virus detection was performed by real-time registration of the source-drain current through the NRs. The detection of the target viral particles was carried out in buffer solutions at the target particles concentration within the range from 107 to 103 viral particles per milliliter (VP/mL). The lowest detectable concentration of the target viral particles was 6 × 10−16 M (corresponding to 104 VP/mL). The use of solutions containing ~109 to 1010 VP/mL resulted in saturation of the sensor surface with the target virions. In the saturation mode, detection was impossible.
