Cell Journal (Dec 2022)

Assessment of Adiponectin and Sperm Function Parameters in Obese and Non-Obese: A Comprehensive Study

  • Marziyeh Pooladi,
  • Mohamadreza Sharifi,
  • Gholamreza Dashti

DOI
https://doi.org/10.22074/cellj.2022.557337.1044
Journal volume & issue
Vol. 24, no. 12
pp. 715 – 722

Abstract

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ObjectiveThe role of adiponectin in sperm function is inconclusive and there is a paucity of evidence. Obesity showsan ambiguous influence on sperm motility, and male subfertility. The aim of this study was to compare the role ofadiponectin and sperm functional parameters among obese and non-obese men.Materials and MethodsIn this comprehensive study, 64 male patients were included, and were classified asnon-obese [body mass index (BMI)25 kg/m2, n=32) groups. Sperm analysis,was conducted using World Health Organization (WHO) 2010 standards. Real-time polymerase chain reaction(PCR) and enzyme-linked immunosorbent assay (ELISA) were used for the analysis of adiponectin gene expressionand protein levels, respectively. Sperm viability was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT test), Acridine orange (AO) test was utilized to detect DNA denaturation, andsperm chromatin dispersion (SCD) technique was used to investigate the fragmentation of DNA.ResultsIn obese men, adiponectin gene expression (P<0.0001) and protein levels (P<0.001) were significantly lowercompared to the non-obese group. Additionally, sperm motility, was significantly lower in the obese group. The rapidprogressive (RP) motility was less in obese men in comparison to the non-obese group (P<0.001). Sperm countand morphology were not significantly different in the two groups. DNA denaturation and DNA fragmentation weresignificantly more frequent in the obese group than in non-obese men (P<0.05) and (P<0.01), respectively. The obesemen showed significantly lower sperm viability compared to the non-obese group (P<0.05).ConclusionThis study showed no significant correlation between the evaluated variables (sperm parameter, spermviability, DNA fragmentation and integrity), and obesity in men. Based on these results, adiponectin may potentially playpositive role in sperm function for acquiring fertility.

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