Topically applied fullerenols protect against radiation dermatitis by scavenging reactive oxygen species
Hanying Yin,
You Gao,
Weiguang Chen,
Chen Tang,
Zihan Zhu,
Kun Li,
Siyu Xia,
Changshun Han,
Xiaoyan Ding,
Fengkai Ruan,
Hanrui Tian,
Changfeng Zhu,
Suyuan Xie,
Zhenghong Zuo,
Lixin Liao,
Chengyong He
Affiliations
Hanying Yin
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
You Gao
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Weiguang Chen
School of Medicine and School of Biomedical Sciences, Huaqiao University
Chen Tang
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Zihan Zhu
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Kun Li
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Siyu Xia
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Changshun Han
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Xiaoyan Ding
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Fengkai Ruan
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Hanrui Tian
State Key Laboratory for Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University
Changfeng Zhu
Xiamen Funano New Materials Technology Co., Ltd.
Suyuan Xie
State Key Laboratory for Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University
Zhenghong Zuo
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Lixin Liao
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Chengyong He
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, The Plastic and Aesthetic Burn Department, The First Affiliated Hospital, Xiamen University
Abstract Adverse skin reactions caused by ionizing radiation are collectively called radiation dermatitis (RD), and the use of nanomedicine is an attractive approach to this condition. Therefore, we designed and large-scale synthesized fullerenols that showed free radical scavenging ability in vitro. Next, we pretreated X-ray-exposed cells with fullerenols. The results showed that pretreatment with fullerenols significantly scavenged intracellular reactive oxygen species (ROS) produced and enhanced the antioxidant capacity, protecting skin cells from X-ray-induced DNA damage and apoptosis. Moreover, we induced RD in mice by applying 30 Gy of X-ray irradiation, followed by treatment with fullerenols. We found that after treatment, the RD scores dropped, and the histological results systematically demonstrated that topically applied fullerenols could reduce radiation-induced skin epidermal thickening, collagen deposition and skin appendage damage and promote hair regeneration after 35 days. Compared with Trolamine cream, a typical RD drug, fullerenols showed superior radiation protection. Overall, the in vitro and in vivo experiments proved that fullerenols agents against RD.
