Journal of Experimental & Clinical Cancer Research (Feb 2011)

Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I

  • Liu Juanjuan,
  • Wang Yanyan,
  • Liang Xiuyun,
  • Gao Jian,
  • Lin Bei,
  • Yan Limei,
  • Gao Lili,
  • Zhang Shulan,
  • Masao Iwamori

DOI
https://doi.org/10.1186/1756-9966-30-15
Journal volume & issue
Vol. 30, no. 1
p. 15

Abstract

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Abstract Background This study aimed to investigate the molecular structural relationship between cell adhesive molecule CD44 and Lewis y antigen, and determine the effects of Lewis y antigen on CD44-mediated adhesion and spreading of ovarian cancer cell line RMG-I and the Lewis y antigen-overexpressed cell line RMG-I-H. Methods The expression of CD44 in RMG-I and RMG-I-H cells before and after treatment of Lewis y monoclonal antibody was detected by immunocytochemistry; the expression of Lewis y antigen and CD44 was detected by Western Blot. The structural relationship between Lewis y antigen and CD44 was determined by immunoprecipitation and confocal laser scanning microscopy. The adhesion and spreading of RMG-I and RMG-I-H cells on hyaluronic acid (HA) were observed. The expression of CD44 mRNA in RMG-I and RMG-I-H cells was detected by real-time RT-PCR. Results Immunocytochemistry revealed that the expression of CD44 was significantly higher in RMG-I-H cells than in RMG-I cells (P P P P P > 0.05). Conclusion Lewis y antigen strengthens CD44-mediated adhesion and spreading of ovarian cancer cells.