Limosilactobacillus fermentum TY-S11 ameliorates hypercholesterolemia via promoting cholesterol excretion and regulating gut microbiota in high-cholesterol diet-fed apolipoprotein E-deficient mice
Yadan Deng,
Jing Wang,
Ran Wang,
Yuying Wang,
Xi Shu,
Pengjie Wang,
Chong Chen,
Feng Zhang
Affiliations
Yadan Deng
Key Laboratory of Conservation, Exploration and Utilization of Southwest Characteristic Bacterial Germplasm Resources, Chongqing Tianyou Dairy Co., Ltd., Chongqing, 401120, China
Jing Wang
Key Laboratory of Conservation, Exploration and Utilization of Southwest Characteristic Bacterial Germplasm Resources, Chongqing Tianyou Dairy Co., Ltd., Chongqing, 401120, China
Ran Wang
Department of Nutrition and Health, Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing, 100190, China
Yuying Wang
Key Laboratory of Conservation, Exploration and Utilization of Southwest Characteristic Bacterial Germplasm Resources, Chongqing Tianyou Dairy Co., Ltd., Chongqing, 401120, China
Xi Shu
Key Laboratory of Conservation, Exploration and Utilization of Southwest Characteristic Bacterial Germplasm Resources, Chongqing Tianyou Dairy Co., Ltd., Chongqing, 401120, China
Pengjie Wang
Department of Nutrition and Health, Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing, 100190, China
Chong Chen
Key Laboratory of Conservation, Exploration and Utilization of Southwest Characteristic Bacterial Germplasm Resources, Chongqing Tianyou Dairy Co., Ltd., Chongqing, 401120, China
Feng Zhang
Key Laboratory of Conservation, Exploration and Utilization of Southwest Characteristic Bacterial Germplasm Resources, Chongqing Tianyou Dairy Co., Ltd., Chongqing, 401120, China; Corresponding author.
Hypercholesterolemia is a metabolic disease characterized by elevated cholesterol level in the blood, which is a risk factor for many diseases. Probiotic intervention may be one of the ways to improve hypercholesterolemia. In this study, three strains with better cholesterol removal ability were selected from 60 strains of lactic acid bacteria, and were orally administered to apolipoprotein E-deficient mice on a high-cholesterol diet. Among the three strains, only Limosilactobacillus fermentum TY-S11, which was isolated from the intestine of a longevity person, significantly improved serum and liver lipid levels in hypercholesterolemic mice. Further study found that L. fermentum TY-S11 promoted the excretion of cholesterol in the feces and inhibited the absorption of cholesterol in the small intestine. As for gut microbiota, the results showed that L. fermentum TY-S11 not only prevented the reduction of diversity caused by high-cholesterol diet, but also increased the contents of short-chain fatty acids in feces. These results confirmed the ameliorative effect of L. fermentum TY-S11 on hypercholesterolemia.
