Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity

Yang Liu; Wanbing Pan; Lingyu Li; Jianquan Gong; Hongjing Dong; Bin Zhang; Jiahao Lin; Zhenjia Zheng

Food Chemistry Advances (Dec 2024)

Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity

Yang Liu,
Wanbing Pan,
Lingyu Li,
Jianquan Gong,
Hongjing Dong,
Bin Zhang,
Jiahao Lin,
Zhenjia Zheng

Affiliations

Yang Liu: Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR China
Wanbing Pan: The Clinical Department, College of Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China; Center of Research and Innovation of Chinese Traditional Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China
Lingyu Li: Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR China
Jianquan Gong: Yantai Baimaishan Biotechnology Co., Ltd., 3 Fushan Street, Yantai 265200, Shandong, PR China
Hongjing Dong: Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, Shandong, PR China
Bin Zhang: Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR China
Jiahao Lin: The Clinical Department, College of Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China; Center of Research and Innovation of Chinese Traditional Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China; Corresponding authors.
Zhenjia Zheng: Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR China; Corresponding authors.

Journal volume & issue: Vol. 5
p. 100784

Abstract

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A novel Arctium lappa L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and in vitro digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm−1, indicating that zinc was successfully complexed with ALP. The stability and in vitro digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-γ, TNF-α, IL-1β and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties.

Published in Food Chemistry Advances

ISSN: 2772-753X (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Food processing and manufacture
Website: https://www.journals.elsevier.com/food-chemistry-advances

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