High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method

Youjun Zeng; Jie Zhou; Wei Sang; Weifu Kong; Junle Qu; Ho-Pui Ho; Kaiming Zhou; Bruce Zhi Gao; Jiajie Chen; Yonghong Shao

doi:10.3389/fchem.2021.801355

Frontiers in Chemistry (Dec 2021)

High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method

Youjun Zeng,
Jie Zhou,
Wei Sang,
Weifu Kong,
Junle Qu,
Ho-Pui Ho,
Kaiming Zhou,
Bruce Zhi Gao,
Jiajie Chen,
Yonghong Shao

Affiliations

Youjun Zeng: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China
Jie Zhou: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China
Wei Sang: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China
Weifu Kong: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China
Junle Qu: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China
Ho-Pui Ho: Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, Hong KongSAR, China
Kaiming Zhou: Aston Institute of Photonic Technologies, Aston University, Birmingham, United Kingdom
Bruce Zhi Gao: Department of Bioengineering and COMSET, Clemson University, Clemson, SC, United States
Jiajie Chen: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China
Yonghong Shao: Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, China

DOI: https://doi.org/10.3389/fchem.2021.801355
Journal volume & issue: Vol. 9

Abstract

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Intensity interrogation surface plasmon resonance (ISPR) sensing has a simple schematic design and is the most widely used surface plasmon resonance technology at present. However, it has relatively low sensitivity, especially for ISPR imaging (ISPRi). In this paper, a new technique for the real-time monitoring of biomolecule binding on sensor surfaces via ISPRi detection is described. The technique is based on the interrogation of the differential value of two intensities at two specific wavelengths from the reflected light spectrum. In addition, we also optimized the selection of dual-wavelength parameters under different circumstances to achieve the highest sensitivity. The new technique achieved a refractive index resolution (RIR) of 2.24 × 10–6 RIU, which is far beyond that of traditional ISPRi technique. Moreover, our new ISPRi technique also realized the real-time detection of high-throughput biomolecular binding. This study is expected to promote the development of faster and more accurate SPRi technologies.

Published in Frontiers in Chemistry

ISSN: 2296-2646 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Chemistry
Website: http://journal.frontiersin.org/journal/chemistry

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