Molecular Genetic Phylogeny of Gossypium L Specimens

Abstract

In the article, the phylogenetic tree of kinship of unique samples created at the Scientific Research Institute of Cotton Selection, Seeding and Cultivation Agrotechnologies and stored in the collection was compiled using molecular markers. 185 primer pairs from TMB, JESPR, GH, NAU, BNL and CIR collections were used, 45 of which turned out to be polymorphic, which allowed to identify 96 polymorphic loci, 20 of which were unique. Phylogenetic analysis of cotton samples based on genotyping using SSR markers divided the samples into 5 different groups. Namangan-34 and Namangan-102 varieties were the most distant from the remaining samples, and these varieties did not differ from each other. Varieties L-175_276 and C-4727 also formed a separate cluster, sample 13 and Sultan variety are related and also form a separate cluster, sample 4 and variety C-7934 also belong. The remaining 29 samples were not significantly different from each other and formed a large cluster, which is called their narrow genetic base.

Keywords

• dna
• phylogeny
• marker
• cotton
• gossypium l
• germplasm
• species
• variety