Effects of High Mobility Group Box 1 on Migration and Expression of Related Proteins in Umbilical Vein Endothelial Cells

Abstract

Purposes The establishment of microcirculation in tissue engineering plays an important role in biomaterials implantation, in which the migration of vascular endothelial cells is the main factor affecting rapid angiogenesis. Tissue and organ injury often leads to inflammation, and high mobility group box 1 (HMGB1) is the initiating factor of inflammation, while its effect on the migration of endothelial cells remains unclear. Methods Human umbilical vein endothelial cells (HUVECs) were extracted and cultured in vitro, and their phenotypes were identified. The concentration gradient of HMGB1 in culture medium was constructed, and the proliferation and migration of HUVECs were detected by CCK-8, scratch test, and Transwell chamber test. The expression of genes and proteins of cell proliferation factor and migration factor in HUVECs was detected by RT-qPCR and Western Blot. Findings Different concentrations of HMGB1 had no significant effect on proliferation of HUVECs, but was concentration-dependent on cell migration. With the increase of HMGB1 concentration, the migration ability of cells was enhanced. The expression of FGF-2 was up-regulated with the increase of HMGB1 concentration. The expression of VEGFA decreased significantly after HUVECs was treated with high HMGB1 concentration. Conclusions HMGB1 can promote the migration of HUVECs through FGF-2, which provides a basis for the study of the effect of HMGB1 on the migration ability of endothelial cells in the future.

Keywords

• human umbilical vein endothelial cells
• high mobility group box 1
• migration
• vascular endothelial growth factor a
• fibroblast growth factor-2