OncoTargets and Therapy (Nov 2019)
Resveratrol Reverses Cigarette Smoke-Induced Urocystic Epithelial–Mesenchymal Transition via Suppression of STAT3 Phosphorylation in SV-HUC-1-Immortalized Human Urothelial Cells
Abstract
Hongliang Sun, Zhiqiang Zhang, Taotao Zhang, Hao Geng, Dongdong Xie, Yi Wang, Demao Ding, Tao Zhang, Dexin Yu Department of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230032, People’s Republic of ChinaCorrespondence: Dexin YuDepartment of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230032, People’s Republic of ChinaTel +86 137 0569 0883Fax +86 551 6386 9522Email [email protected]: Bladder cancer is a malignant tumor of the urinary tract, and cigarette smoke (CS) is closely related to tumorigenesis. Resveratrol, a plant-derived bioactive nutrient, possesses multiple anticancer effects. However, the mechanism of CS-induced tumorigenesis is still not clear. The role of resveratrol in CS-meditated bladder cancer development has not been reported.Methods: MTT assay showed the toxicity of cigarette smoke extract (CSE) on the cell viability of SV-HUC-1 cells. Western blotting detected the expression levels of related proteins. Transwell migration or invasion assay evaluated the capacity of cell migration or invasion after treatment. Wound-healing assay revealed the effect of cell migratory capacity. The cell cycle was detected by flow cytometry.Results: Our study demonstrated that CSE-triggered epithelial–mesenchymal transition (EMT) in SV-HUC-1-immortalized human urothelial cells via the STAT3/TWIST1 pathway. Furthermore, the results showed resveratrol effectively inhibited STAT3 phosphorylation, thus reversed EMT triggered by CSE. Meanwhile, the cell proliferation was also suppressed.Conclusion: In conclusion, inhibition of the STAT3 in CSE-induced EMT on bladder cancer may be a promising cancer treatment target for suppression by resveratrol.Keywords: bladder cancer, cigarette smoke, resveratrol, STAT3/TWIST1
