Mitochondrial Metabolism Characterization of Four Different Fish Cell Lines

Abstract

Mitochondria are a crucial cellular organelle that organizes a wide range of biological processes, from energy production and calcium homeostasis to cell proliferation, differentiation, and death as well as inflammation. Mitochondria also support immune cell phenotypes and function. The aim of this communication is to evaluate the mitochondrial status and plasticity of four fish cell lines: SAF-1 (derived from gilthead sea bream skin), DLB-1 (derived from European sea bass brain), FuB-1 (established from mummichog brain), and PLHC-1 (a topminnow hepatocellular carcinoma). A mitochondrial stress assay was performed to compare the bioenergetic capabilities of these four fish cell lines as well as the importance of choosing the correct cell line when performing different in vitro studies.

Keywords

• cell culture
• energetic reserve
• mitochondrial stress
• respiration