CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

Krzysztof Chylinski; Maria Hubmann; Ruth E. Hanna; Connor Yanchus; Georg Michlits; Esther C. H. Uijttewaal; John Doench; Daniel Schramek; Ulrich Elling

doi:10.1038/s41467-019-13403-y

Nature Communications (Nov 2019)

CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

Krzysztof Chylinski,
Maria Hubmann,
Ruth E. Hanna,
Connor Yanchus,
Georg Michlits,
Esther C. H. Uijttewaal,
John Doench,
Daniel Schramek,
Ulrich Elling

Affiliations

Krzysztof Chylinski: Vienna Biocenter Core Facilities, Vienna Biocenter (VBC)
Maria Hubmann: Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna Biocenter (VBC)
Ruth E. Hanna: Broad Institute of MIT and Harvard
Connor Yanchus: Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Georg Michlits: Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna Biocenter (VBC)
Esther C. H. Uijttewaal: Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna Biocenter (VBC)
John Doench: Broad Institute of MIT and Harvard
Daniel Schramek: Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital
Ulrich Elling: Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna Biocenter (VBC)

DOI: https://doi.org/10.1038/s41467-019-13403-y
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Inducible genome editing systems often suffer from leakiness or reduced activity. Here the authors develop CRISPR-Switch, a Cre recombinase ON/OFF-controlled sgRNA cassette that allows consecutive editing of two loci.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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