A xanthine oxidase inhibit activity component from biotransformation of cholesterol by Streptomyces cellulosae WHX1301

Abstract

Steroids are one of the most widely used groups of medicines presently. There are some steroid drugs that have acquired with the transformation of microorganism. It's indispensability to screen the strain that is able to utilize steroids to generate new products. This study has screened a transformation strain WHX1301 that have ability to convert cholesterol. Based on the 16S rRNA gene sequence comparison, the isolate WHX1301 has been demonstrated to most similar as Streptomyces cellulosae. Separation and purification of transformation product were identifying by NMR and ESI-MS. The major of product was 2,7-dihydroxycholesterol, and the by-product were 7-Hydroxycholestane-3,5-diene, Cholesterane-3,5-diene. Fortunately, 2,7-dihydroxycholesterol has inhibitory activity against xanthine oxidase with a 34.8% inhibition rate at a concentration of 20 μg/ml. Using the resting cells of Streptomyces cellulosae WHX1301 to transform cholesterol, the product yield can reach 76%. Present paper is the first report regarding the microbial transformation of steroids by Streptomyces cellulosae.

Keywords

• Biotransformation
• Cholesterol
• Streptomyces cellulosae
• Resting cell
• Xanthine oxidase