Induction of Mutation for Traits Related to Pod in Rapeseed (Brassica Napus L.) Cultivars Using Gamma Rays

Abstract

The grains of cultivars Rapeseed Sarigol and RGS003 were irradiated with gamma rays at doses 0, 800, 1000 and 1200 Gary (GY). For M2 generation, the grain of each plant was cultivated in a separate row and all plants were individually investigated during cultivation. According to the observed changes in agronomic traits such as pod length, the number of pods and flowering during M2 generation, 18 and 12 candidate mutant lines were obtained from Sarigol and RGSOO3, respectively. The differences among gamma rays doses were not statistically significant for seed sterility, while they were significant for pollen sterility at the level of 1%. Maximum pollen sterility was observed in 800 GY. Induced mutations in RGS003 and Sariglo cultivars were mostly obtained in 1200 and 800 GY, respectively. The M3 mutant lines in both Rapeseed cultivars contained a variation on traits such as the number of pod per the main stem, the number of pod per plant, pod length, the number of grain per pod, grain weight and flowering duration.

Keywords

• gamma rays
• induced mutation
• rapeseed (brassica napus l.)
• pod length
• mutant