Antitumor Potential of Green Synthesized ZnONPs Using Root Extract of <i>Withania somnifera</i> against Human Breast Cancer Cell Line
Kollur Shiva Prasad,
Shashanka K Prasad,
Ravindra Veerapur,
Ghada Lamraoui,
Ashwini Prasad,
M. N. Nagendra Prasad,
Sandeep Kumar Singh,
Najat Marraiki,
Asad Syed,
Chandan Shivamallu
Affiliations
Kollur Shiva Prasad
Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka 570026, India
Shashanka K Prasad
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570015, India
Ravindra Veerapur
Department of Metallurgy and Materials Engineering, Malawi Institute of Technology, Malawi University of Science and Technology, P.O. Box 5916, Limbe, Malawi
Ghada Lamraoui
Nature and Life Sciences, Earth and Universe Sciences, University of Tlemcen, Tlemcen, Algeria
Ashwini Prasad
Department of Microbiology and Tissue Culture, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570015, India
M. N. Nagendra Prasad
Department of Biotechnology, JSS Science and Technolgy University, Mysuru, Karnataka 570004, India
Sandeep Kumar Singh
Indian Scientific Education and Technology (ISET) Foundation, Lucknow 226002, India
Najat Marraiki
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Asad Syed
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Chandan Shivamallu
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570015, India
Herein we report the synthesis of zinc oxide nanoparticles (ZnONPs) using Withania somnifera root extract (WSE) as an effective chelating agent. The microscopic techniques viz., X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), and selected area electron diffraction (SAED) were employed to analyze the as-obtained ZnONPs. The crystalline planes observed from the XRD pattern agrees with the hexagonal wurtzite structure of the as-prepared ZnONPs. The aggregations and agglomerations observed in the SEM images indicated that the size of the as-prepared ZnONPs was between 30 and 43 nm. The interplanar distance between the lattice fringes observed in the HRTEM image was found to be 0.253 nm, which is in good agreement with the (100) plane obtained in the XRD pattern. Furthermore, the anti-breast cancer cytotoxic evaluation was carried out using the MCF-7 cell line, and the results showed significant cytotoxic effects in a dose-dependent manner.
