Frontiers in Plant Science (Mar 2023)
Generation of new rice germplasms with low amylose content by CRISPR/CAS9-targeted mutagenesis of the FLOURY ENDOSPERM 2 gene
- Xiaohong Song,
- Xiaohong Song,
- Zhihui Chen,
- Zhihui Chen,
- Xi Du,
- Xi Du,
- Bin Li,
- Bin Li,
- Yunyan Fei,
- Yunyan Fei,
- Yajun Tao,
- Yajun Tao,
- Fangquan Wang,
- Fangquan Wang,
- Yang Xu,
- Yang Xu,
- Wenqi Li,
- Wenqi Li,
- Jun Wang,
- Jun Wang,
- Guohua Liang,
- Guohua Liang,
- Yong Zhou,
- Yong Zhou,
- Xiaoli Tan,
- Yulong Li,
- Jie Yang,
- Jie Yang,
- Jie Yang
Affiliations
- Xiaohong Song
- School of Life Science, Jiangsu University, Zhenjiang, Jiangsu, China
- Xiaohong Song
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Zhihui Chen
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Zhihui Chen
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Xi Du
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Xi Du
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Bin Li
- School of Life Science, Jiangsu University, Zhenjiang, Jiangsu, China
- Bin Li
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Yunyan Fei
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Yunyan Fei
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Yajun Tao
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Yajun Tao
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Fangquan Wang
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Fangquan Wang
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Yang Xu
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Yang Xu
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Wenqi Li
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Wenqi Li
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Jun Wang
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Jun Wang
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Guohua Liang
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Guohua Liang
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Yong Zhou
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Yong Zhou
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- Xiaoli Tan
- School of Life Science, Jiangsu University, Zhenjiang, Jiangsu, China
- Yulong Li
- School of Life Science, Jiangsu University, Zhenjiang, Jiangsu, China
- Jie Yang
- School of Life Science, Jiangsu University, Zhenjiang, Jiangsu, China
- Jie Yang
- Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs, Nanjing, Jiangsu, China
- Jie Yang
- Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China
- DOI
- https://doi.org/10.3389/fpls.2023.1138523
- Journal volume & issue
-
Vol. 14
Abstract
FLOURY ENDOSPERM 2 (FLO2), encoding a tetratricopeptide repeat domain (TPR)-containing protein located in the nucleus, is considered to be a regulatory protein that controls the biosynthesis of seed storage substances. The diversity of flo2 allele is attributable for the variations in grain appearance, amylose content (AC), and physicochemical properties, influencing the eating and cooking quality (ECQ) of rice. In this study, we used CRISPR/Cas9 to introduce loss-of-function mutations into the FLOURY ENDOSPERM 2 gene in Suken118 (SK118), a widely cultivated elite japonica rice variety in Jiangsu, China. Physiochemical analyses of the flo2 mutants were congruent with previous studies, exhibiting lowered AC and viscosity, risen gel consistency (GC) and gelatinization temperature (GT) values, which were all instrumental to the improvement of ECQ. However, the wrinkled opaque appearance and the decrease in grain width, grain thickness and grain weight imply trade-offs in grain yield. Despite the ex-ante estimation for low yielding, the superior ECQ in these novel genotypes generated by using genome editing approach may have the potential for formulating high value specialty food.
Keywords
- FLOURY ENDOSPERM 2
- amylose content (AC)
- eating and cooking quality (ECQ)
- japonica rice
- CRISPR/Cas9
- genome editing