Sex-Based Differences in Multilocus Heterozygosity in Wild Boar from Spain

Abstract

Wild boar (Sus scrofa) populations are increasing worldwide, leading to health, economic and conservation issues. Assessing genetic structure and diversity can aid in the effective monitoring and management of wild boar populations. Processes related to sexual selection and behavioral differences between sexes support the importance of considering sex in measuring genetic diversity. In this study, we investigated the genetic diversity of wild boar in southwestern Spain by comparing multilocus heterozygosity (MLH) in males and females. We collected tissue samples from 142 culled individuals and 146 fetuses during routine hunting activities and used 16 microsatellite markers to quantify MLH. Paternity analyses were conducted to infer the genotypes of reproductive males. Our results indicated that the sampled individuals constituted a unique polygynandrous population without clear genetic structure. We found that males tended to exhibit lower MLH than females, with reproductive males showing significantly lower MLH than females. We discuss the selection and demographic processes that might explain our results. We highlight the importance of sex-balanced culling for population control, as well as the use of sex-balanced samples for monitoring genetic diversities.

Keywords

• genetic diversity
• microsatellite markers
• paternity analysis
• population management
• sexual selection
• <i>Sus scrofa</i>